Escherichia coli K99 pili are composed of one subunit species

Effect of sodium acetate on expression of K99 pili by Escherichia coli.

Sodium acetate suppressed K99 production in Escherichia coli strains cultured on a minimal medium, as determined by seroagglutination and enzyme-linked immunosorbent assay. The greatest suppression occurred when the medium contained both sodium acetate and glucose. Glucose alone did not suppress K99 production.

Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.

A new method for the extraction and purification of K99 pili from enterotoxigenic Escherichia coli and their characterization.

It was found that K99 pili from enterotoxigenic Escherichia coli (of bovine origin) could be extracted by treatment with 3M-KSCN solution. The K99 pili were purified by preparative isoelectric focusing to apparent homogeneity as judged by the presence of a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the molecular weight of this component was calculated to be 12 60...

Analysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.

A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.

The role of mannose sensitive and mannose resistant pili of Escherichia coli in urinary tract infection

  In order to study the relationship between hrmolysin / ashesins of Escherichia coli and occurrence of urinary tract infection ( UTI), the capacity of 570 isolated E.coli in agglutination of human or guinea pig erythrocytes and their lysis were tested. These isolates were obtained from the urine of patients with bacteriuria and from the stool of healthy people. Three patterns of hemagglutinat...